Variation in the Bluetongue Virus Neutralization Protein VP2
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چکیده
منابع مشابه
Variation in the bluetongue virus neutralization protein VP2.
To determine the extent and nature of the antigenic variation of four U.S.A. serotypes of bluetongue virus (BTV), the complete nucleotide sequence was determined for cDNA clones representing the L2 dsRNA of BTV serotype 13, the gene that codes for the outer capsid neutralization antigen (VP2). The predicted amino acid sequence of the protein was compared with the VP2 sequences of the U.S.A. ser...
متن کاملBluetongue virus serotype 17 sequence variation associated with neutralization.
Bluetongue virus (BTV) is an insect-transmitted orbivirus of importance to the cattle and sheep industry. The VP2 protein, encoded by L2, contains neutralizing epitopes. Previously, a panel of neutralizing monoclonal antibodies (MAbs) to the BTV serotype 17 (BTV-17) prototype strain was generated and it was determined that the neutralization domain consists of three overlapping epitopes. Over 3...
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Seven neutralizing monoclonal antibodies (N-MAbs) were generated to an Australian isolate of bluetongue virus serotype 1 [BTV-1 (Aust)]. At least five of the N-MAbs were specific for epitopes on the outer coat protein VP2 and one was capable of binding SDS-treated protein in a Western blot. Six of the N-MAb panel bound and four of these neutralized BTV-1 (South Africa). None of the N-MAbs neutr...
متن کاملCloning and Expression of VP2 Outer Coat Protein Gene of Bluetongue Serotype1 Virus, Indian Isolate
Bluetongue (BT) is a viral disease causing morbidity and mortality in sheep, cattle and wild ruminants, including deer, sambar and bluebull. In this study we present the internal gene of outer coat protein, VP2 of bluetongue virus serotype 1 of Indian isolate was amplified from cDNA synthesized through RT-PCR using total RNA with specific forward and reverse primers. The PCR product was cloned ...
متن کاملBluetongue virus coat protein VP2 contains sialic acid-binding domains, and VP5 resembles enveloped virus fusion proteins.
Bluetongue virus (BTV) is transmitted by blood-feeding insects (Culicoides sp.) and causes hemorrhagic diseases in livestock. BTV is a nonenveloped, double-stranded RNA (dsRNA) virus with two capsids: a well-studied, stable core enclosing the dsRNA genome and a highly unstable, poorly studied coat responsible for host cell attachment and entry. Here, based on cryo-electron microscopy (cryoEM), ...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1987
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-68-11-2967